The role of fs(1)K10 in the localization of the mRNA of the TGF alpha homolog gurken within the Drosophila oocyte

Mech Dev. 1995 Jun;51(2-3):183-92. doi: 10.1016/0925-4773(95)00363-0.

Abstract

A critical step in Drosophila dorsoventral patterning is the movement of gurken mRNA from the anterior cortex of the oocyte to the oocyte's anterodorsal corner at stage 8 of oogenesis. Such movement is dependent on fs(1)K10. It has been proposed that fs(1)K10 mediates gurken mRNA movement by down-regulating gurken mRNA levels, thus ensuring that gurken mRNA does not saturate its receptors located in the oocyte's anterodorsal corner. In contradiction to this model, we show here--both genetically and immunocytochemically--that GRK protein levels are lower in the anterodorsal region of fs(1)K10 mutant oocytes than in the anterodorsal region of fs(1)K10+ oocytes. From this and other data, we propose a more direct role for fs(1)K10 in the gurken mRNA localization process.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Polarity
  • Down-Regulation
  • Drosophila / genetics
  • Drosophila / growth & development
  • Drosophila / metabolism*
  • Drosophila Proteins*
  • Female
  • Genes, Insect
  • Insect Hormones / genetics*
  • Male
  • Mutation
  • Oocytes / metabolism*
  • Oogenesis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Transforming Growth Factor alpha / genetics
  • Transforming Growth Factors / genetics*

Substances

  • Drosophila Proteins
  • Insect Hormones
  • RNA, Messenger
  • Transforming Growth Factor alpha
  • grk protein, Drosophila
  • Transforming Growth Factors