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, 28 (6), 1149-54

Transfer of non-T-DNA Portions of the Agrobacterium Tumefaciens Ti Plasmid pTiA6 From the Left Terminus of TL-DNA

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Transfer of non-T-DNA Portions of the Agrobacterium Tumefaciens Ti Plasmid pTiA6 From the Left Terminus of TL-DNA

V Ramanathan et al. Plant Mol Biol.

Abstract

We introduced a plant selection marker, nptII, to the left of border A in the Agrobacterium Ti plasmid pTiA6. Infection of tobacco leaf discs with the modified Agrobacterium strain gave rise to kanamycin-resistant calli which grew in a hormone-dependent manner. Southern hybridization analysis of DNA isolated from four transformants indicated initiation of DNA transfer at or near border A and absence of T-DNA sequences. These results demonstrate that DNA transfer events starting at a left border on a native Ti plasmid and moving away from the T-DNA region occur and that they can be detected by designing a suitable selection strategy.

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