FISH in genome research and molecular diagnostics

Curr Opin Genet Dev. 1995 Jun;5(3):304-8. doi: 10.1016/0959-437x(95)80043-3.


Fluorescence in situ hybridization (FISH) has profoundly altered the aspect of genome research and molecular diagnostics. Deletions of only a few kilobases can be detected by hybridizing probes to naked DNA fibers. Loss or gain of chromosomal material in tumor cells can be visualized using comparative genome hybridization. Further diversification of FISH application will result from new ultrasensitive detection techniques.

Publication types

  • Review

MeSH terms

  • Chromosome Aberrations
  • Congenital Abnormalities / genetics
  • DNA / analysis
  • Genetic Diseases, Inborn / diagnosis
  • Genetic Diseases, Inborn / genetics
  • Genome, Human
  • Human Genome Project
  • Humans
  • In Situ Hybridization, Fluorescence / methods
  • In Situ Hybridization, Fluorescence / trends*
  • RNA / analysis


  • RNA
  • DNA