Quantitative Locus Analysis of Airway Hyperresponsiveness in A/J and C57BL/6J Mice

Nat Genet. 1995 Oct;11(2):150-4. doi: 10.1038/ng1095-150.

Abstract

Airway hyperresponsiveness is a key characteristic of human asthma and a marker for asthma-like conditions in animals. F1 mice derived from A/J and C57BL/6J display a phenotype which resembles the asthma-like phenotype of the A/J mice. Since airway responsiveness failed to segregate as a mendelian trait, we show significant linkage at two loci, Bhr1 (lod = 3.0) and Bhr2 (lod = 3.7) on chromosomes 2 and 15. A third locus, Bhr3 (lod = 2.83), maps to chromosome 17. Each of these loci maps near candidate loci implicated in the pathobiology of asthma. Our study represents the first linkages established through a genome-wide survey of airway hyperresponsiveness in any mammal.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Asthma / genetics*
  • Asthma / physiopathology*
  • Chromosome Mapping*
  • Crosses, Genetic
  • DNA / analysis
  • DNA / isolation & purification
  • Female
  • Genetic Linkage
  • Genotype
  • Humans
  • Kidney / metabolism
  • Lod Score
  • Lung / drug effects
  • Lung / physiology*
  • Male
  • Mammals
  • Methacholine Chloride / pharmacology
  • Mice
  • Mice, Inbred A
  • Mice, Inbred C57BL
  • Parasympathomimetics / pharmacology
  • Phenotype
  • Plethysmography
  • Polymerase Chain Reaction
  • Respiratory Function Tests*

Substances

  • Parasympathomimetics
  • Methacholine Chloride
  • DNA