Expression of GABA receptor rho 1 and rho 2 subunits in the retina and brain of the rat

Eur J Neurosci. 1995 Jul 1;7(7):1495-501. doi: 10.1111/j.1460-9568.1995.tb01144.x.


We have investigated the distribution of GABA receptor rho 1 and rho 2 subunits in the rat central nervous system. Cloning of rat rho 1 and rho 2 cDNA fragments revealed similarities to the corresponding human sequences of 99% (rho 1) and 88% (rho 2) at the protein level. Whereas the human rho 2 subunit has no consensus sequence for phosphorylation by protein kinase C, the cytoplasmic loop of the rat sequence contains two such sites. Use of the polymerase chain reaction with reverse-transcribed total RNA (RT-PCR) from different brain tissues revealed that transcript for the rho 1 subunit was present in the retina only. The rho 2 mRNA was detected in all brain regions, with the highest level of expression in the retina. In situ hybridization of retinal sections revealed that rho 1 and rho 2 transcripts are present in the inner nuclear layer. RT-PCR and in situ hybridization of isolated retinal cells showed that both rho subunits are present in rod bipolar cells. Since these cells express bicuculline-insensitive GABA receptors, our results further support the idea that rho subunits are part of the GABAc receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Cloning, Molecular
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Oligonucleotide Probes / genetics
  • Polymerase Chain Reaction
  • Rats
  • Rats, Wistar
  • Receptors, GABA / metabolism*
  • Retina / cytology
  • Retina / metabolism*
  • Retinal Rod Photoreceptor Cells / cytology
  • Retinal Rod Photoreceptor Cells / metabolism
  • Tissue Distribution


  • Oligonucleotide Probes
  • Receptors, GABA

Associated data

  • GENBANK/S79783
  • GENBANK/S79784