Intrathymically expressed c-kit ligand (stem cell factor) is a major factor driving expansion of very immature thymocytes in vivo

Immunity. 1995 Sep;3(3):313-9. doi: 10.1016/1074-7613(95)90116-7.


To investigate the role of the receptor-type tyrosine kinase, c-kit and its ligand, stem cell factor (SCF) in T cell development, we analyzed c-kit (W/W) and SCF (SI/SI) deficient mice. We also engrafted wild-type or SCF-deficient fetal thymi onto wild-type recipient mice and analyzed the rate of proliferation by in vivo bromodeoxyuridine labeling. The results show that the most immature thymocyte compartment defined as CD3-CD4-CD8- is significantly reduced in SI/SI grafts and W/W thymi compared with wild-type counterparts. Also, the expansion rate of these immature thymocytes in SI/SI graft is reduced by -50%. These experiments provide direct evidence for an important role for c-kit-SCF interactions in expansion of very early thymocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bromodeoxyuridine / metabolism
  • CD4 Antigens / analysis
  • CD8 Antigens / analysis
  • Cell Movement
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Stem Cell Factor / genetics
  • Stem Cell Factor / physiology*
  • T-Lymphocytes / physiology*


  • CD4 Antigens
  • CD8 Antigens
  • Stem Cell Factor
  • Bromodeoxyuridine