The cytoplasmic and transmembrane domains of MHC class II beta chains deliver distinct signals required for MHC class II-mediated B cell activation

Immunity. 1995 Sep;3(3):349-58. doi: 10.1016/1074-7613(95)90119-1.


Class II-mediated signals play potential roles in B cell activation and antigen presentation. The regions of the class II molecule participating in B cell signaling are incompletely defined. Our prior analysis of structural requirements of the cytoplasmic domain of A beta revealed that only the eight membrane-proximal residues are required for signaling. Here, we report that the sequence and position of two of these are critical, and present direct evidence that the A beta transmembrane domain is also involved in signaling, via a pathway distinct from the cytoplasmic domain. These results demonstrate that specific regions in both the cytoplasmic and transmembrane domains of the class II molecule have distinct signaling functions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Cyclic AMP / biosynthesis
  • Histocompatibility Antigens Class II / chemistry
  • Histocompatibility Antigens Class II / physiology*
  • Lymphocyte Activation*
  • Mice
  • Molecular Sequence Data
  • Structure-Activity Relationship
  • Transfection


  • Histocompatibility Antigens Class II
  • Cyclic AMP