Abstract
Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphatases / physiology*
-
Animals
-
Carrier Proteins / pharmacology
-
Carrier Proteins / physiology*
-
Golgi Apparatus / enzymology*
-
Golgi Apparatus / ultrastructure
-
Golgi Matrix Proteins
-
Intracellular Membranes / physiology*
-
Membrane Proteins / pharmacology
-
Microscopy, Electron
-
Mitosis / physiology*
-
Molecular Weight
-
N-Ethylmaleimide-Sensitive Proteins
-
Rats
-
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
-
Vesicular Transport Proteins*
-
Xenopus
Substances
-
Carrier Proteins
-
Golgi Matrix Proteins
-
Membrane Proteins
-
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
-
Vesicular Transport Proteins
-
vesicular transport factor p115
-
Adenosine Triphosphatases
-
N-Ethylmaleimide-Sensitive Proteins
-
Nsf protein, rat