An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments

Cell. 1995 Sep 22;82(6):905-14. doi: 10.1016/0092-8674(95)90270-8.


Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / physiology*
  • Animals
  • Carrier Proteins / pharmacology
  • Carrier Proteins / physiology*
  • Golgi Apparatus / enzymology*
  • Golgi Apparatus / ultrastructure
  • Golgi Matrix Proteins
  • Intracellular Membranes / physiology*
  • Membrane Proteins / pharmacology
  • Microscopy, Electron
  • Mitosis / physiology*
  • Molecular Weight
  • N-Ethylmaleimide-Sensitive Proteins
  • Rats
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins*
  • Xenopus


  • Carrier Proteins
  • Golgi Matrix Proteins
  • Membrane Proteins
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins
  • vesicular transport factor p115
  • Adenosine Triphosphatases
  • N-Ethylmaleimide-Sensitive Proteins
  • Nsf protein, rat