A possible role of TGF-beta in the formation of malignant effusions

Clin Immunol Immunopathol. 1995 Oct;77(1):27-32. doi: 10.1016/0090-1229(95)90133-7.


The detailed mechanisms underlying the formation of malignant effusions are incompletely defined. In order to determine whether transforming growth factor-beta (TGF-beta) would contribute to the formation of malignant effusions, we investigated the effect of TGF-beta on the morphology, growth, and permeability of human mesothelial cells, which are thought to serve as a permeability barrier in the pleuroperitoneal cavities. Treatment of the mesothelial cells with a TGF-beta dose ranging from 0.1 to 10 ng/ml for 96 hr induced distinct morphologic changes in the cells. Each cell increased in size as did the volume of the intercellular spaces. TGF-beta also significantly inhibited the growth of mesothelial cells at a concentration ranging from 0.1 to 10 ng/ml. This growth inhibition was blocked completely by the addition of anti-TGF-beta antibody. Treatment of the mesothelial cells with 2.0 ng/ml TGF-beta significantly increased the permeability of a mesothelial cell monolayer as assessed by a FITC-albumin permeability assay. In our clinical analysis using 10 effusion samples obtained from patients with various types of carcinoma cells, considerable level of TGF-beta could be detected by ELISA, ranged from 0.90 to 8.75 ng/ml. Our data suggest that TGF-beta plays an important role in the formation of malignant effusions through structural and functional damage to the mesothelial cells. Malignant effusions may accumulate in the pleuroperitoneal cavity as a result of the mesothelial cell damage caused by this cytokine which is released from disseminated cancer cells.

MeSH terms

  • Adult
  • Aged
  • Ascites / pathology*
  • Cell Division
  • Cell Membrane Permeability / drug effects
  • Cells, Cultured
  • Epithelial Cells*
  • Female
  • Growth Inhibitors / pharmacology
  • Humans
  • In Vitro Techniques
  • Male
  • Middle Aged
  • Omentum
  • Pleural Effusion, Malignant / pathology*
  • Transforming Growth Factor beta / physiology*


  • Growth Inhibitors
  • Transforming Growth Factor beta