Enhanced adeno-associated virus vector expression by adenovirus protein-cationic liposome complex. A novel and high efficient way to introduce foreign DNA into endothelial cells

H Zhou; G Zeng; X Zhu; J Tang; G Chen; Q Huang; T Peng; B Hu

Enhanced adeno-associated virus vector expression by adenovirus protein-cationic liposome complex. A novel and high efficient way to introduce foreign DNA into endothelial cells

Chin Med J (Engl). 1995 May;108(5):332-7.

Authors

H Zhou¹, G Zeng, X Zhu, J Tang, G Chen, Q Huang, T Peng, B Hu

Affiliation

¹ Cardiovascular Research Institute, Beijing Medical University.

PMID: 7555232

Abstract

Adeno-associated virus vector (AAV), a non-pathogenic integrating vector, can integrate on chromosome 19 in humans. But its gene transfer efficiency is quite low. In this study we combined adenovirus (Adv-5) capsid protein or the Fiber protein of Adv with liposome, termed adenosome (adenovirus protein-cationic liposome complex). This complex can bring AAV/CMV-LacZ to the endothelial cells, and improve the efficiency of gene transfer. It may be a novel, specific, stable and safe gene delivery system and will be widely used in human gene therapy.

MeSH terms

Adenoviruses, Human / genetics*
Base Sequence
Capsid / genetics
Cells, Cultured
Endothelium, Vascular* / cytology
Genes, Reporter*
Genetic Vectors*
Humans
Liposomes
Molecular Sequence Data
Muscle, Smooth, Vascular* / cytology
Transfection*
Viral Proteins

Substances

Liposomes
Viral Proteins