IL-1 alpha is constitutively expressed in the rat intestinal epithelial cell line IEC-6

Exp Cell Res. 1995 Oct;220(2):298-303. doi: 10.1006/excr.1995.1319.


The rat small intestinal epithelial cell line, IEC-6, was examined for the presence of IL-1 mRNAs using the reverse transcription/polymerase chain reaction method. IL-1 alpha but not IL-1 beta transcripts were detected in plastic adherent cells. The levels of IL-1 alpha transcripts were similar in cells cultured at different densities. IL-1 activity was not detected, by bioassay, in the culture supernatant of the cells, nor was it membrane associated. IL-1 activity was detected in cell lysates, although its measurement was made difficult by the presence of an inhibitor of the bioassay. Intracellular IL-1 alpha was detectable using Western blots. Interleukin-1 receptor antagonist mRNA was also detectable in plastic adherent cells. Treatment with TGF-beta 1, IL-1 beta, IL-6, TNF-alpha, or combinations of any two of these cytokines failed to induce the secretion of the IL-1 alpha or to significantly change the levels of mRNA. The IEC-6 cell line is similar to other epithelial cells in having intracellular pools of IL-1 alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Western
  • Cell Adhesion
  • Cell Line
  • DNA Primers
  • Epithelium / metabolism
  • Gene Expression*
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / analysis
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / pharmacology
  • Intestines
  • Kinetics
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Rats
  • Sialoglycoproteins / biosynthesis
  • T-Lymphocytes, Helper-Inducer / drug effects
  • T-Lymphocytes, Helper-Inducer / immunology


  • DNA Primers
  • Il1rn protein, mouse
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • RNA, Messenger
  • Sialoglycoproteins