Induction of vascular endothelial growth factor expression in synovial fibroblasts by prostaglandin E and interleukin-1: a potential mechanism for inflammatory angiogenesis

FEBS Lett. 1995 Sep 18;372(1):83-7. doi: 10.1016/0014-5793(95)00956-a.


Inflammatory mediators such as prostaglandin E2 (PGE2) and interleukin-1 (IL-1) induce angiogenesis by yet undefined mechanisms. We demonstrate that PGE2 and IL-1 induces the expression of vascular endothelial growth factor (VEGF), a selective angiogenic factor by rheumatoid synovial fibroblast cells. Transcripts for the EP1 and EP2 subtypes of PGE receptors are expressed in synovial fibroblasts. Activators of protein kinase A pathway stimulated the expression of VEGF whereas down-regulation of protein kinase C did not influence the PGE effect, suggesting that signalling from the EP2 receptor via the protein kinase A pathway is important. The induction of VEGF expression by PGE2 and interleukin-1 alpha may be an important mechanism in inflammatory angiogenesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arthritis, Rheumatoid / metabolism*
  • Bacterial Toxins / pharmacology
  • Base Sequence
  • Colforsin / pharmacology
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • DNA Primers
  • Endothelial Growth Factors / genetics*
  • Fibroblasts / metabolism
  • Gene Expression Regulation*
  • Humans
  • Interleukin-1 / pharmacology*
  • Lymphokines / genetics*
  • Molecular Sequence Data
  • Neovascularization, Pathologic / etiology*
  • Prostaglandins E / pharmacology*
  • Protein Kinase C / metabolism
  • Receptors, Prostaglandin E / genetics
  • Signal Transduction
  • Synovial Fluid / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors


  • Bacterial Toxins
  • DNA Primers
  • Endothelial Growth Factors
  • Interleukin-1
  • Lymphokines
  • Prostaglandins E
  • Receptors, Prostaglandin E
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Colforsin
  • Cyclic AMP-Dependent Protein Kinases
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate