Deleted in oral cancer-1 (doc-1), a novel oral tumor suppressor gene

FASEB J. 1995 Oct;9(13):1362-70. doi: 10.1096/fasebj.9.13.7557027.


We have identified, isolated, and partially characterized doc-1, a novel cDNA sequence whose activity is consistent with a suppressor of hamster oral carcinogenesis. Doc-1 is an evolutionarily conserved gene exhibiting loss of heterozygosity and marked reduction in expression in malignant hamster oral keratinocytes. The full-length doc-1 cDNA encodes an 87 amino acid product that shows a significant homology to one of the seven novel genes induced in mouse fibroblasts by TNF-alpha. Transfection of the full-length doc-1 cDNA into malignant hamster oral keratinocytes alters the behavior of the recipients in terms of morphology, growth rate, and anchorage-independent growth, suggesting reversion of transformation phenotypes. We propose that doc-1 is a novel tumor suppressor gene in oral cancer development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary / genetics
  • Gene Deletion
  • Genes, Tumor Suppressor*
  • Keratinocytes / physiology
  • Molecular Sequence Data
  • Mouth Neoplasms / genetics*
  • Proteins / genetics*
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins*


  • CDK2AP1 protein, human
  • DNA, Complementary
  • Proteins
  • Tumor Suppressor Proteins

Associated data

  • GENBANK/U10249