Effects of mutations and deletions on expression of the Enterobacter cloacae ompX gene

G de Kort; P van der Bent-Klootwijk; R A van Boxtel; J A van de Klundert

doi:10.1016/0378-1097(95)00184-7

Effects of mutations and deletions on expression of the Enterobacter cloacae ompX gene

FEMS Microbiol Lett. 1995 Jul 15;130(1):63-8. doi: 10.1016/0378-1097(95)00184-7.

Authors

G de Kort¹, P van der Bent-Klootwijk, R A van Boxtel, J A van de Klundert

Affiliation

¹ Department of Medical Microbiology, University Hospital, Leiden, The Netherlands.

PMID: 7557297
DOI: 10.1016/0378-1097(95)00184-7

Abstract

The Enterobacter cloacae outer membrane protein OmpX is involved in resistance to beta-lactams, and possesses virulence characteristics. To gain more insight into the genetic elements that are important for OmpX expression, several mutations were introduced at, and immediately upstream of, the N-terminus of the OmpX coding sequence. These mutations enabled us to delete the 5' untranslated region and the signal peptide coding sequence. The former led to decreased ompX expression, indicating an unexpected and hitherto unexplained role for this region. Deletion of the signal peptide coding sequence blocked transport across the cytoplasmic membrane, indicating that translocation of OmpX across the cytoplasmic membrane is mediated by the general secretory pathway.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Outer Membrane Proteins / biosynthesis*
Bacterial Outer Membrane Proteins / metabolism
Biological Transport
Cell Membrane / metabolism
Enterobacter cloacae / genetics*
Escherichia coli Proteins*
Gene Expression Regulation, Bacterial
Genes, Bacterial / genetics*
Hydrolases*
Mutagenesis
Protein Sorting Signals / genetics
RNA, Messenger / biosynthesis
Sequence Deletion / genetics*

Substances

Bacterial Outer Membrane Proteins
Escherichia coli Proteins
Protein Sorting Signals
RNA, Messenger
OmpX protein, E coli
Hydrolases