Molecular analysis of a 66-kDa protein associated with the outer membrane of Lyme disease Borrelia

FEMS Microbiol Lett. 1995 Sep 1;131(2):139-45. doi: 10.1111/j.1574-6968.1995.tb07768.x.

Abstract

A 66-kDa protein (p66) associated with the outer membrane of Lyme disease Borrelia was analysed at the molecular level. The chromosomal genes encoding p66 in B. burgdorferi B31, B. afzelii ACAI, and B. garinii Ip90 were sequenced. Database searches revealed that the p66 gene sequences were homologous to a previously reported gene fragment of unknown function. The deduced amino acid sequences of p66 in different Lyme disease borreliae were 92-94% identical and had no homologs in the databases. Proteolytic cleavage patterns of p66 and a computer-predicted single trans-membrane helix suggested the presence of surface-exposed epitopes on the C-terminus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / genetics*
  • Base Sequence
  • Borrelia / genetics*
  • Borrelia / pathogenicity
  • Borrelia burgdorferi Group / genetics
  • Borrelia burgdorferi Group / pathogenicity
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Humans
  • Lyme Disease / etiology
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Molecular Weight
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • Bacterial Outer Membrane Proteins
  • DNA, Bacterial

Associated data

  • GENBANK/L32596
  • GENBANK/X87725
  • GENBANK/X87726
  • GENBANK/X87727