Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus

Gene. 1995 Sep 22;163(1):65-8. doi: 10.1016/0378-1119(95)00387-l.


A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus / enzymology
  • Bacillus / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA Polymerase I / biosynthesis
  • DNA Polymerase I / genetics*
  • DNA Polymerase II / biosynthesis
  • DNA Polymerase II / genetics
  • Genes, Bacterial*
  • Genome, Bacterial
  • Genomic Library
  • Geobacillus stearothermophilus / enzymology*
  • Geobacillus stearothermophilus / genetics*
  • Molecular Sequence Data
  • Plasmids
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Homology, Amino Acid


  • DNA Polymerase I
  • DNA Polymerase II

Associated data

  • GENBANK/U23149