Serum-induced collagen gel contraction

Graefes Arch Clin Exp Ophthalmol. 1995 Jul;233(7):430-4. doi: 10.1007/BF00180947.


Purpose: To understand the molecular events underlying disease-related vitreous gel contraction, the effect of serum components on collagen was investigated.

Methods: Bovine vitreous or dermal collagen was incubated with a mixture of transglutaminase (TG; factor XIIIa) and fibronectin (FN), and the biochemical changes of collagen were monitored by gel electrophoresis. In addition, serum-induced changes in the volume of the collagen gel were monitored.

Results: Gel electrophoresis revealed a new high-molecular-weight band (M(r) 240,000) presumably due to intermolecular cross-links of collagen peptides and FN. The serum components also were shown to cause a significant decrease in the volume of the collagen gel. CONCLUSION. Collagen gel contraction could be attributed to the collagen-FN-collagen cross-links catalyzed by TG.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Collagen / drug effects
  • Collagen / isolation & purification
  • Collagen / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Fibronectins / metabolism
  • Fibronectins / pharmacology*
  • Molecular Weight
  • Skin / chemistry
  • Transglutaminases / pharmacology*
  • Vitreous Body / chemistry


  • Fibronectins
  • Collagen
  • Transglutaminases