Cloning and chromosomal localization of the human A2b adenosine receptor gene (ADORA2B) and its pseudogene

Genomics. 1995 May 20;27(2):374-6. doi: 10.1006/geno.1995.1061.


To determine the chromosomal localization of the human A2b adenosine receptor, the corresponding genomic clone was isolated and used as a probe for fluorescence in situ hybridization to metaphase chromosomes. Partial sequence analysis of the A2b gene (AD-ORA2B) revealed an intron that interrupted the coding region corresponding to the second intracellular loop similar to that reported for A1 and A2a adenosine receptor genes. A pseudogene for the A2b receptor was also identified; it exhibited 79% identity to the A2b adenosine receptor cDNA coding sequence and contained multiple deletions, point mutations, and frame shifts and two in-frame stops. These changes would result in the inability to encode a functional receptor. The genomic clones were utilized to localize the A2b receptor to chromosome 17p12 and the A2b pseudogene to chromosome 1q32.

MeSH terms

  • Chromosome Mapping
  • Chromosomes, Human, Pair 17*
  • Cloning, Molecular
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Pseudogenes*
  • Receptors, Purinergic P1 / genetics*


  • Receptors, Purinergic P1

Associated data

  • GENBANK/L38716
  • GENBANK/X68487