The cytotoxic HLA-DQ3 reactive human hybridoma antibody 4166 that may distinguish DQ7 + 8 from DQ9

Hum Immunol. 1995 Apr;42(4):281-8. doi: 10.1016/0198-8859(94)00111-3.


The human cytotoxic hybridoma antibody 4166 (IgM kappa) was generated by fusing an in vitro EBV-transformed B-LCL from a multiparous woman with the mouse-human heteromyeloma fusion partner CB-F7. In microcytotoxicity and IIF tests with B-LCLs as target cells, the mAb 4166 was specific for DQ3 (= DQ7 + 8 + 9). However, when used for DQ typing of class-II-positive PBMCs, 4166 could be rendered functionally specific for DQ7 + 8 and did not react with DQ9+ PBMCs. Binding of mAb 4166 to DQ8-positive cells was efficiently blocked by several allotype-specific mAbs recognizing DQ8. Other HLA class-II-specific mAbs were unable to inhibit. With the use of mAb 4166, it is possible to discriminate DQ7 + 8 from DQ9 in serologic DQ typing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Antigen-Antibody Reactions
  • Binding, Competitive
  • Cytotoxicity Tests, Immunologic
  • Female
  • HLA-DQ Antigens / immunology*
  • Histocompatibility Testing
  • Humans
  • Hybridomas / metabolism
  • Immunomagnetic Separation
  • Leukocytes, Mononuclear / immunology
  • Mice
  • Molecular Sequence Data


  • Antibodies, Monoclonal
  • HLA-DQ Antigens
  • HLA-DQ3 antigen