Evaluation of commercial antisera for Shigella serogrouping

J Clin Microbiol. 1995 Aug;33(8):1997-2001. doi: 10.1128/jcm.33.8.1997-2001.1995.

Abstract

Shigella serogrouping antisera from six companies (Becton Dickinson, Denka, Difco, Murex, Roach, and Sanofi-Pasteur) intended for the slide agglutination test and those of the Wellcolex Colour Shigella latex agglutination test were evaluated to identify quality products for Shigella identification. Forty-six reference Shigella strains (one for each serotype and species), 50 clinical strains (21 S. flexneri, 21 S. sonnei, 4 S. dysenteriae, 4 S. boydii) representing the most prevalent species and serotypes encountered in Quebec, and 9 non-Shigella strains were tested according to the manufacturers' instructions. A 3+ reaction (> or = 75% agglutination) was considered positive for the slide agglutination tests. Sensitivity varied from 47% (Roach) to 94% (Difco). For the 105 strains tested, accuracy ranged from 53% (Roach) to 91% (Wellcolex). Specificity varied from 97 to 100% for group A antisera, from 96 to 100% for group B antisera, from 88 to 100% for group C antisera, and from 95 to 99% for group D antisera. The costs of reagents required to test one strain varied from $3.50 to $13.20 (in Canadian dollars). In conclusion, Roach reagents proved to be unsatisfactory for Shigella serogrouping. Among those from the remaining companies, the Denka, Difco, and Wellcolex reagents met a performance standard of 90% accuracy.

Publication types

  • Comparative Study

MeSH terms

  • Agglutination Tests / methods
  • Agglutination Tests / statistics & numerical data
  • Antibodies, Bacterial*
  • Antibody Specificity
  • Dysentery, Bacillary / diagnosis
  • Dysentery, Bacillary / microbiology
  • Evaluation Studies as Topic
  • Humans
  • Indicators and Reagents
  • Latex Fixation Tests / methods
  • Latex Fixation Tests / statistics & numerical data
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serotyping / methods*
  • Serotyping / statistics & numerical data
  • Shigella / classification*
  • Shigella / immunology*
  • Shigella boydii / classification
  • Shigella boydii / immunology
  • Shigella dysenteriae / classification
  • Shigella dysenteriae / immunology
  • Shigella flexneri / classification
  • Shigella flexneri / immunology
  • Shigella sonnei / classification
  • Shigella sonnei / immunology
  • Species Specificity

Substances

  • Antibodies, Bacterial
  • Indicators and Reagents