Identification of an endotoxin and IFN-inducible cDNA: possible identification of a novel protein family

J Leukoc Biol. 1995 Oct;58(4):477-84. doi: 10.1002/jlb.58.4.477.

Abstract

The response of macrophages to agents such as lipopolysaccharide (LPS) and interferon (IFN) includes the transcriptional activation of numerous genes. We have used the method of differential screening of a RAW 264.7 macrophage cell line cDNA library to isolate and characterize LPS-induced messages. One such message, LRG-47, is induced by LPS, IFN-gamma, and IFN-alpha/beta, but not by a panel of other cytokines or pharmacological activating agents. LRG-47 is homologous to two other IFN-gamma-induced genes, IRG-47 and Mg21. The LRG-47 sequence is approximately 33% identical and 52% similar to both these putative protein products. All three putative proteins, particularly Mg21, bear homology to a T cell product, Tgtp, induced by T cell receptor cross-linking. The three macrophage-derived proteins share areas of homology with GTP-binding proteins, are approximately 415 amino acids in length, and have similar kinetics of induction by IFN-gamma. This suggests that these genes may be members of a new family of IFN-inducible proteins.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / metabolism
  • Base Sequence
  • Cells, Cultured
  • Clone Cells
  • DNA, Complementary / analysis*
  • DNA, Complementary / biosynthesis*
  • DNA, Complementary / genetics
  • GTP-Binding Proteins / chemistry
  • Interferons / pharmacology*
  • Kinetics
  • Lipopolysaccharides / pharmacology*
  • Macrophages / drug effects*
  • Macrophages / metabolism*
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Recombinant Proteins / pharmacology
  • Sequence Homology, Amino Acid
  • Stimulation, Chemical

Substances

  • DNA, Complementary
  • Ifi1 protein, mouse
  • Lipopolysaccharides
  • RNA, Messenger
  • Recombinant Proteins
  • Interferons
  • GTP-Binding Proteins

Associated data

  • GENBANK/U19119