Dose-dependent gingival overgrowth induced by cyclosporin in rats

J Periodontol. 1995 Jul;66(7):594-8. doi: 10.1902/jop.1995.66.7.594.


This study evaluated the effect of dosage on severity of cyclosporin-A (CSA) induced gingival overgrowth. Eighty (80) male Sprague-Dawley rats were randomly distributed into 4 groups. Rats in each group daily received CSA in mineral oil by gastric feeding at dosages of 0 (control), 3, 10, and 30 mg/kg, respectively, for 6 weeks. Stone models of the mandibular incisal region were obtained biweekly and were used for analysis of the gingival dimensions. Animals were sacrificed at the end of week 6 and tissue sections were processed for histopathologic evaluations. Animals were sacrificed at the end of week 6 and tissue sections were processed for histopathologic evaluation Gingival overgrowth including bucco-lingual and mesio-distal width and vertical height were significantly increased with increasing CSA dosage. Furthermore, the gingival dimensions displayed a positive linear relation to dosage and treatment duration. The histopathologic evaluation revealed a granulomatous tissue wedging the tooth-gingival interface in the 3 mg/kg group. This tissue had reached exuberant size in the 10 and 30 mg/kg groups. In summary, the analysis of gingival dimensions the histopathologic evaluation shows a dose-dependent effect on the severity of CSA-induced gingival overgrowth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Connective Tissue / drug effects
  • Connective Tissue / pathology
  • Cyclosporine / administration & dosage
  • Cyclosporine / adverse effects*
  • Dental Models
  • Dose-Response Relationship, Drug
  • Epithelium / drug effects
  • Epithelium / pathology
  • Fibrosis
  • Gingival Hyperplasia / chemically induced*
  • Gingival Hyperplasia / pathology
  • Granulation Tissue / drug effects
  • Granulation Tissue / pathology
  • Granuloma / chemically induced
  • Granuloma / pathology
  • Immunosuppressive Agents / administration & dosage
  • Immunosuppressive Agents / adverse effects*
  • Incisor
  • Intubation, Gastrointestinal / instrumentation
  • Male
  • Mandible
  • Multivariate Analysis
  • Periodontal Ligament / drug effects
  • Periodontal Ligament / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors


  • Immunosuppressive Agents
  • Cyclosporine