Abstract
The role of Pseudomonas aeruginosa exo-enzymes was evaluated in a murine model of endogenous bacteraemia in which the bacteria invaded the bloodstream after oral dosing. Although an elastase mutant PAO-E64 was as virulent as its parent strain PAO1, an exo-enzyme S-deficient mutant, DG1-ExS5 and alkaline protease mutants PAKS-16, PAKS-17, PAKS-19, were less virulent than their parent strains, DG1 and PAKS-1, respectively (p < 0.01). Thus exo-enzyme S and alkaline protease, but not elastase, appear to contribute to the pathogenicity of P. aeruginosa in this model.
MeSH terms
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Animals
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Bacteremia / microbiology*
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Bacterial Proteins*
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Disease Models, Animal
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Endopeptidases / genetics
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Endopeptidases / metabolism*
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Male
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Metalloendopeptidases / metabolism
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Mice
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Mutation
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Poly(ADP-ribose) Polymerases / genetics
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Poly(ADP-ribose) Polymerases / metabolism*
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Pseudomonas Infections / microbiology*
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / pathogenicity
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Specific Pathogen-Free Organisms
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Virulence
Substances
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Bacterial Proteins
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Poly(ADP-ribose) Polymerases
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Endopeptidases
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Metalloendopeptidases
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pseudolysin, Pseudomonas aeruginosa