The influence of exo-enzyme S and proteases on endogenous Pseudomonas aeruginosa bacteraemia in mice

J Med Microbiol. 1995 Oct;43(4):258-61. doi: 10.1099/00222615-43-4-258.


The role of Pseudomonas aeruginosa exo-enzymes was evaluated in a murine model of endogenous bacteraemia in which the bacteria invaded the bloodstream after oral dosing. Although an elastase mutant PAO-E64 was as virulent as its parent strain PAO1, an exo-enzyme S-deficient mutant, DG1-ExS5 and alkaline protease mutants PAKS-16, PAKS-17, PAKS-19, were less virulent than their parent strains, DG1 and PAKS-1, respectively (p < 0.01). Thus exo-enzyme S and alkaline protease, but not elastase, appear to contribute to the pathogenicity of P. aeruginosa in this model.

MeSH terms

  • Animals
  • Bacteremia / microbiology*
  • Bacterial Proteins*
  • Disease Models, Animal
  • Endopeptidases / genetics
  • Endopeptidases / metabolism*
  • Male
  • Metalloendopeptidases / metabolism
  • Mice
  • Mutation
  • Poly(ADP-ribose) Polymerases / genetics
  • Poly(ADP-ribose) Polymerases / metabolism*
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / enzymology*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / pathogenicity
  • Specific Pathogen-Free Organisms
  • Virulence


  • Bacterial Proteins
  • Poly(ADP-ribose) Polymerases
  • Endopeptidases
  • Metalloendopeptidases
  • pseudolysin, Pseudomonas aeruginosa