Alterations of the p16 (MTS1) gene in testicular, ovarian, and endometrial malignancies

J Urol. 1995 Nov;154(5):1954-7.


Purpose: The p16 (MTS1) gene codes for a cyclin-dependent kinase inhibitor and may be a new tumor suppressor gene. It is frequently mutated in a variety of cell lines established from tumors. This is the first report of screening for alteration of the p16 gene in testicular, ovarian and endometrial malignancies.

Materials and methods: We examined alterations of p16 in 78 primary genital tumors (42 testicular, 21 ovarian and 15 endometrial cancers) and mononuclear cells from 2 patients with Lynch syndrome II as well as 5 testicular tumor cell lines by single-strand conformation polymorphism (SSCP) and Southern blot hybridization.

Results: The DNA from the p16 gene of 2 testicular tumors (5%), an ovarian cancer (4%) and a testicular tumor cell line (20%) had altered migration in gel electrophoresis as shown by SSCP. Analysis of DNA sequence of these samples revealed a polymorphism at codon 140. Southern blot hybridization detected neither deletions nor rearrangements of the p16 gene in any of the samples.

Conclusions: Taken together, these results suggest that p16 alterations probably are not important for tumorigenesis of testicular, ovarian and endometrial tumors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Endometrial Neoplasms / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Tumor Suppressor / genetics*
  • Humans
  • Male
  • Molecular Sequence Data
  • Ovarian Neoplasms / genetics*
  • Polymorphism, Single-Stranded Conformational
  • Testicular Neoplasms / genetics*