Zeon-1, a member of a new maize retrotransposon family

Mol Gen Genet. 1995 Aug 30;248(4):471-80. doi: 10.1007/BF02191647.

Abstract

We have previously shown that the tandemly duplicated 27 kDa maize storage protein locus underwent mitotic rearrangement to yield a single-copy allele in isolates of the inbred line A188. This rearrangement contains a new LTR retrotransposon, designated Zeon-1. This middle repetitive element of 7313 bp had two long terminal repeats, a primer binding site, a polypurine tract and a gag-related open reading frame of 375 amino acids. Transcripts of the gag-related region were detected by the polymerase chain reaction (PCR) in certain maize tissues, and Western blots detected the gag-related protein in the same tissues. Moreover, the product of this mitotic rearrangement was shown to contain the same insertion site and 3' LTR as Zeon-1, suggesting that this rearrangement occurs with unusual precision. Zeon elements were found to be present in teosinte and not present in the Gramineae wheat, barley, sorghum and rye.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Plant
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Humans
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Retroelements*
  • Sequence Homology, Amino Acid
  • Zea mays / genetics*

Substances

  • DNA, Plant
  • Plant Proteins
  • Retroelements
  • Zeon1 gag protein, Zea mays

Associated data

  • GENBANK/U11059