Signalling Downstream of Activated Mammalian Notch

Nature. 1995 Sep 28;377(6547):355-8. doi: 10.1038/377355a0.

Abstract

Notch belongs to a family of transmembrane proteins that are widely conserved from flies to vertebrates and are thought to be involved in cell-fate decisions. In Drosophila, the Suppressor of hairless (Su(H)) gene and genes of the Enhancer of split (E(Spl)) complex, which encode proteins of the basic helix-loop-helix type have been implicated in the Notch signalling pathway. Mammalian homologues of E(Spl), such as the mouse Hairy enhancer of split (HES-1), have been isolated. Both HES-1 and the intracellular domain of murine Notch (mNotch) are able to block MyoD-induced myogenesis. Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-J kappa (refs 8,9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Line
  • DNA-Binding Proteins / metabolism
  • Drosophila
  • Drosophila Proteins*
  • HeLa Cells
  • Homeodomain Proteins / genetics
  • Humans
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein
  • Membrane Proteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Mutagenesis
  • Nuclear Proteins*
  • Oligodeoxyribonucleotides
  • Promoter Regions, Genetic
  • Receptors, Notch
  • Repressor Proteins / genetics
  • Transcription Factor HES-1
  • Transcription Factors / metabolism*
  • Transcriptional Activation*
  • Transfection

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Hes1 protein, mouse
  • Homeodomain Proteins
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein
  • Membrane Proteins
  • N protein, Drosophila
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • RBPJ protein, human
  • Rbpj protein, mouse
  • Receptors, Notch
  • Repressor Proteins
  • Su(H) protein, Drosophila
  • Transcription Factor HES-1
  • Transcription Factors
  • HES1 protein, human