A YAC contig map of the human genome

Nature. 1995 Sep 28;377(6547 Suppl):175-297. doi: 10.1038/377175a0.


A yeast artificial chromosome library containing 33,000 clones with an average insert size of one megabase of human genomic DNA was extensively analysed by several different procedures for detecting overlaps and positional information. We developed an analysis strategy that resulted, after confirmatory tests, in a YAC contig map reliably covering about 75% of the human genome in 225 contigs having an average size of about ten megabases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping* / methods
  • Chromosomes, Artificial, Yeast*
  • DNA Fingerprinting
  • Genetic Markers
  • Genome, Human*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Nucleic Acid Hybridization
  • Sequence Tagged Sites


  • Genetic Markers