Protection against mycoplasma infection using expression-library immunization

Nature. 1995 Oct 19;377(6550):632-5. doi: 10.1038/377632a0.


As is evident from the human immunodeficiency virus epidemic, there is no systematic method for producing a vaccine. Genetic immunization is a new approach to vaccine production that has many of the advantages of live/attenuated pathogens but no risk of infection. It involves introducing DNA encoding a pathogen protein into host cells and has shown promise in several disease models. Here we describe a new method for vaccine development, expression-library immunization, which makes use of the technique of genetic immunization and the fact that all the antigens of a pathogen are encoded in its DNA. An expression library of pathogen DNA is used to immunize a host thereby producing the effects of antigen presentation of a live vaccine without the risk. We show that even partial expression libraries made from the DNA of Mycoplasma pulmonis, a natural pathogen in rodents, provide protection against challenge from the pathogen. Expression library immunization may prove to be a general method for vaccination against any pathogen.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Base Sequence
  • DNA, Bacterial / administration & dosage*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / immunology
  • DNA, Recombinant / administration & dosage*
  • DNA, Recombinant / genetics
  • DNA, Recombinant / immunology
  • Gene Library
  • Humans
  • Lung / immunology
  • Lung / pathology
  • Lung Diseases / immunology
  • Lung Diseases / microbiology
  • Lung Diseases / pathology
  • Lung Diseases / prevention & control
  • Mice
  • Molecular Sequence Data
  • Mycoplasma Infections / immunology
  • Mycoplasma Infections / prevention & control*


  • Antibodies, Bacterial
  • DNA, Bacterial
  • DNA, Recombinant