Evaluation by histology, immunohistology and PCR of protocollized renal biopsies 1 week post-transplant in relation to subsequent rejection episodes

Nephrol Dial Transplant. 1995;10(6):847-54.


Renal biopsies were performed 1 week following renal transplantation at a time without clinical evidence of rejection in 43 patients (13 females, mean age 48 years range 18-60 and 30 males, mean age 43 years range 17-59 years). Thirty-six biopsies were available for histological or immunohistochemical analysis. Immunohistochemical analyses were performed with monoclonal antibodies against leukocytes (CD45), monocytes (WT14), complement factor 3 (C3), T-cells (Leu4), T-cell receptor alpha beta and gamma delta, tumour necrosis factor alpha (TNF alpha), IL-2 receptor (IL2-R, TAC), intercellular adhesion molecule-1 (ICAM1) and HLA-DR. The slides were scored semiquantitatively with the observers having no knowledge of clinical or patient data. TNF alpha and IL-2R were also measured by quantative PCR. None of the studied parameters correlated to delayed graft function or graft loss. Histological analysis showed that both focal interstitial infiltrate (18/35) and tubular basement membrane disruption (11/35) were followed by a higher incidence of subsequent rejection (P = 0.03 and 0.02 respectively). Also positivity for WT14 around tubuli (P = 0.02) was associated with subsequent occurrence of rejection. The intensity of staining of ICAM-1 on PTC as well as TAC on proximal tubular cells was associated with the number of subsequent rejection episodes. The association between the IL-2 receptor and subsequent rejection was also found applying PCR to the tissue specimens. We conclude that the presence of focal interstitial infiltrates and tubulitis in 1-week biopsies from well-functioning grafts carries an increased risk of subsequent rejection.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Isopropylmalate Synthase / analysis
  • Adolescent
  • Adult
  • Aged
  • Analysis of Variance
  • Base Sequence
  • Biopsy
  • Cytokines / analysis
  • Female
  • Follow-Up Studies
  • Fungal Proteins / analysis
  • Graft Rejection / immunology
  • Graft Rejection / pathology*
  • Humans
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1 / analysis
  • Kidney / immunology
  • Kidney / pathology*
  • Kidney Diseases / surgery
  • Kidney Transplantation / immunology
  • Kidney Transplantation / pathology*
  • Leukocyte Common Antigens / analysis
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Prospective Studies
  • Saccharomyces cerevisiae Proteins*
  • Time Factors
  • Transplantation, Homologous


  • Cytokines
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Intercellular Adhesion Molecule-1
  • 2-Isopropylmalate Synthase
  • LEU4 protein, S cerevisiae
  • Leukocyte Common Antigens