Involvement of extracellular matrix in acetylcholine receptor epsilon-subunit gene expression at the rat neuromuscular junction

Neurosci Lett. 1995 Jun 23;193(1):33-6. doi: 10.1016/0304-3940(95)11661-f.


During neuromuscular development, the nerve induces the expression of acetylcholine receptor (AChR) epsilon-subunit gene selectively in synaptic myonuclei. Here we show that even after elimination of neural effects by denervation, synaptic expression of epsilon-subunit transcripts is maintained for > 4 months. In contrast, after damage of the extracellular matrix (ECM) by treatment with proteolytic enzymes, epsilon-subunit mRNA is significantly reduced within less than 1 day, indicating a role of ECM in the regulation of AChR subunit transcripts at the synapse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Extracellular Matrix / physiology*
  • Gene Expression / genetics*
  • Muscle, Skeletal / physiology
  • Neuromuscular Junction / physiology*
  • Presynaptic Terminals / metabolism
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cholinergic / genetics*
  • Time Factors


  • RNA, Messenger
  • Receptors, Cholinergic