Molecular cloning of a cDNA with a novel domain present in the tre-2 oncogene and the yeast cell cycle regulators BUB2 and cdc16

Oncogene. 1995 Sep 21;11(6):1139-48.


In an effort to identify genes that are differentially regulated during mast cell development, subtracted cDNA prepared from wild-type murine P815 mastocytoma cells and a P815 subline that exhibits properties of mast cell differentiation was used to screen mast cell cDNA libraries. Several known mast cell-specific cDNAs were isolated including mast cell carboxypeptidase A (MC-CPA), murine mast cell protease-5 (MMCP-5), and gp49. A novel cDNA, designated Tbc1, was identified that showed differential expression in the two mast cell lines. The amino acid sequence predicted from the cDNA contains a 200 amino acid domain that is homologous to regions in the tre-2 oncogene and the yeast regulators of mitosis, BUB2 and cdc16. The N-terminal region contains a number of cysteine and histidine residues, potentially encoding a zinc finger domain. Tbc1 is a nuclear protein and is expressed in highest levels in hematopoietic cells, testis and kidney. Within these tissues, expression of Tbc1 is cell- and stage-specific. Based on sequence similarity, pattern of expression and subcellular localization, Tbc1 may play a role in the cell cycle and differentiation of various tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Base Sequence
  • Cell Cycle
  • Cell Cycle Proteins / genetics*
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • DNA-Binding Proteins / genetics
  • Endopeptidases*
  • Erythroid-Specific DNA-Binding Factors
  • Fungal Proteins / genetics*
  • GTPase-Activating Proteins
  • Mast-Cell Sarcoma / pathology
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / analysis
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / physiology
  • Oncogene Proteins*
  • Oncogene Proteins, Fusion / genetics*
  • Oncogenes*
  • Proto-Oncogene Proteins
  • Rabbits
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics
  • Tumor Cells, Cultured
  • Ubiquitin Thiolesterase


  • Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
  • BUB2 protein, S cerevisiae
  • CDC16 protein, S cerevisiae
  • Cdc16 protein, mouse
  • Cell Cycle Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • Fungal Proteins
  • GTPase-Activating Proteins
  • Nuclear Proteins
  • Oncogene Proteins
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Proteins
  • Saccharomyces cerevisiae Proteins
  • Tbc1d1 protein, mouse
  • Transcription Factors
  • Endopeptidases
  • USP6 protein, human
  • Ubiquitin Thiolesterase

Associated data

  • GENBANK/U33005