Genotypic analysis of multiple loci in somatic cells by whole genome amplification

Nucleic Acids Res. 1995 Sep 11;23(17):3488-92. doi: 10.1093/nar/23.17.3488.


To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / genetics*
  • Alleles
  • DNA, Neoplasm / genetics*
  • Esophageal Neoplasms / genetics*
  • Genetic Markers
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Sequence Deletion


  • DNA, Neoplasm
  • Genetic Markers