Development of a Species-Specific Probe for Mycobacterium Xenopi

Res Microbiol. Mar-Apr 1995;146(3):237-43. doi: 10.1016/0923-2508(96)80279-8.

Abstract

In Europe, Mycobacterium xenopi is a frequently isolated species among opportunist mycobacteria, and represents one of the main agents of pulmonary infection due to non-tuberculous mycobacteria. Conventional identification of mycobacteria is a time-consuming and laborious process. In this study, we propose a rapid and simple method for the identification of M. xenopi using polymerase chain reaction. The amplified product consists of a specific probe, present in all 38 M. xenopi strains tested, which could not be amplified and did not present cross-hybridization with a set of 110 strains belonging to 23 other mycobacterial species. The probe was cloned and sequenced. Comparison with data bases revealed no significant homologies with previously described sequences.

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • DNA Probes / chemistry*
  • DNA, Bacterial / chemistry*
  • Electrophoresis, Agar Gel
  • In Vitro Techniques
  • Molecular Sequence Data
  • Mycobacterium / genetics
  • Mycobacterium / isolation & purification*
  • Polymerase Chain Reaction / methods*

Substances

  • DNA Probes
  • DNA, Bacterial

Associated data

  • GENBANK/S79713