Serial analysis of gene expression

Science. 1995 Oct 20;270(5235):484-7. doi: 10.1126/science.270.5235.484.


The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • DNA, Complementary / genetics
  • Gene Expression*
  • Gene Library
  • Genetic Techniques*
  • Humans
  • Molecular Sequence Data
  • Pancreas / enzymology*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics*


  • DNA, Complementary
  • RNA, Messenger
  • DNA Restriction Enzymes

Associated data

  • GENBANK/D00306
  • GENBANK/J05582
  • GENBANK/M11949
  • GENBANK/M18692
  • GENBANK/M22612
  • GENBANK/M24400
  • GENBANK/M27602
  • GENBANK/M28443
  • GENBANK/M29366
  • GENBANK/M55994
  • GENBANK/M81057
  • GENBANK/M91159
  • GENBANK/M93285
  • GENBANK/R45808
  • GENBANK/S94541
  • GENBANK/U19977
  • GENBANK/X01448
  • GENBANK/X15759
  • GENBANK/X54457
  • GENBANK/X61499
  • GENBANK/X67318
  • GENBANK/Z11692