The amino-terminal portion of pro-brain natriuretic peptide (Pro-BNP) circulates in human plasma

Biochem Biophys Res Commun. 1995 Sep 25;214(3):1175-83. doi: 10.1006/bbrc.1995.2410.


Using an antiserum raised in rabbits to a synthetic human brain natriuretic peptide (BNP) N-terminal fragment [ProBNP(1-13)], a single large molecular weight (MW) N-terminal form of ProBNP has been identified in human plasma. Sep-pak extracts of plasma, drawn from patients with congestive heart failure and subjected to size exclusion and reverse phase high pressure liquid chromatography (HPLC) coupled to radioimmunoassay (RIA) revealed a single large immunoreactive (ir-) peak which was not detected in assays of BNP-32 [ProBNP(77-108)]. This material has a MW of 8600--similar to that expected for the complete N-terminal portion of ProBNP(1-76). Previously reported high MW BNP forms, cross reacting with BNP-32 antisera, were not detected using the N-terminal antisera, indicating that this material is unlikely to be intact ProBNP(1-108). In patients with congestive heart failure plasma ir-levels of N-terminal ProBNP were greatly raised compared to normal subjects and were up to ninefold higher than ir-BNP-32 values.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Chromatography, High Pressure Liquid / methods
  • Female
  • Heart Failure / blood*
  • Humans
  • Male
  • Middle Aged
  • Natriuretic Peptide, Brain
  • Nerve Tissue Proteins / blood*
  • Nerve Tissue Proteins / isolation & purification
  • Peptide Fragments / blood*
  • Peptide Fragments / isolation & purification
  • Rabbits / immunology
  • Radioimmunoassay / methods
  • Reference Values
  • Regression Analysis


  • Nerve Tissue Proteins
  • Peptide Fragments
  • Natriuretic Peptide, Brain