Instability at the H-ras minisatellite is associated with the spontaneous abortion of the embryo

Biochem Biophys Res Commun. 1995 Sep 25;214(3):788-92. doi: 10.1006/bbrc.1995.2355.

Abstract

Recently we have shown that microsatellite instability (MI) is a detectable phenomenon in aborted embryonic tissues. In the present study we investigated if instability is also detectable in a minisatellite located at the 3'-end of the H-ras proto-oncogene, affecting either the repetition number of the 28-bp core generating larger or smaller alleles or its sequence creating a detectable restriction fragment length polymorphism (RFLP). Among 30 aborted embryonic tissues, alterations at the repetition number of the core were found in 3 (10%) while point mutations were detected in 7 (23%) cases. These results indicate that structural alterations of the H-ras minisatellite may be associated with the rejection of the embryo.

MeSH terms

  • Abortion, Spontaneous / genetics*
  • Base Sequence
  • DNA Primers
  • DNA, Satellite / genetics*
  • Deoxyribonucleases, Type II Site-Specific
  • Embryo, Mammalian
  • Female
  • Genes, ras*
  • Humans
  • Molecular Sequence Data
  • Point Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • Pregnancy
  • Proto-Oncogenes*
  • Repetitive Sequences, Nucleic Acid

Substances

  • DNA Primers
  • DNA, Satellite
  • CCWGG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific