Cloning, subcellular localization and expression of CHL1, a subunit of magnesium-chelatase in soybean

Biochem Biophys Res Commun. 1995 Oct 4;215(1):422-8. doi: 10.1006/bbrc.1995.2481.

Abstract

Mg-insertion is the first committed step in chlorophyll synthesis and is catalyzed by Mg-chelatase. In photosynthetic bacteria, bchI gene product was suggested to be a subunit of Mg-chelatase. We isolated a bchI homolog from a soybean cDNA library and designated it as chlI. CHLI consisted of 421 amino acid residues and the sequence exhibited a high similarity to other BchI homologs. CHLI contained an ATP-binding motif found in other BchI homologs. CHLI was localized in the soluble fraction in soybean chloroplasts, suggesting that it was a stromal subunit of Mg-chelatase. chlI mRNA in cell culture (SB-P) of soybean was reversibly induced by light.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Animals
  • Arabidopsis / enzymology
  • Binding Sites
  • Blotting, Northern
  • Blotting, Western
  • Chloroplasts / enzymology
  • Cloning, Molecular*
  • Consensus Sequence
  • DNA, Complementary / chemistry
  • Euglena gracilis / enzymology
  • Gene Expression*
  • Lyases / chemistry
  • Lyases / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger / metabolism
  • Rhodobacter capsulatus / enzymology
  • Sequence Alignment
  • Soybeans / enzymology*
  • Subcellular Fractions / enzymology*

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Adenosine Triphosphate
  • Lyases
  • magnesium chelatase

Associated data

  • GENBANK/D45857