Developmentally regulated mouse gene NK10 encodes a zinc finger repressor protein with differential DNA-binding domains

DNA Cell Biol. 1995 Nov;14(11):971-81. doi: 10.1089/dna.1995.14.971.

Abstract

Using oligonucleotides complementary to the conserved inter-finger region of a variety of previously described zinc finger-encoding genes, a novel mouse gene was cloned and characterized. The gene is localized on chromosome 8 and comprises five exons. Its corresponding mRNA is developmentally regulated in various tissues and includes an open reading frame encoding a protein of 72,422 daltons. It shares amino-terminal homologies with human KRAB (or FPB) boxes, and contains 13 zinc fingers of the C2-H2 type. The NK10 KRAB domains exhibit repressing activity when tested in GAL4 fusion protein assays. Cloning of putative target sequences revealed that the individual domains differentially contribute to zinc-dependent target DNA binding.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Exons / genetics
  • Gene Expression Regulation, Developmental*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • Recombinant Fusion Proteins / biosynthesis
  • Repressor Proteins / analysis
  • Repressor Proteins / biosynthesis
  • Repressor Proteins / genetics*
  • Sequence Analysis, DNA
  • Zinc Fingers*

Substances

  • DNA-Binding Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Zfp90 protein, mouse

Associated data

  • GENBANK/E14049
  • GENBANK/X79828