Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learning

Neuron. 1995 Oct;15(4):829-42. doi: 10.1016/0896-6273(95)90174-4.


The linotte (lio) gene was identified in a screen for mutations that disrupted 3 hr memory after olfactory associative learning, without affecting the perception of odors or electroshock. The mutagenesis yielded a transposon-tagged gene disruption, which allowed rapid cloning of genomic DNA. The lio transcription unit was identified via rescue of the lio1 learning/memory defect by induced expression of a lio+ transgene in adults. The perception of odors or electroshock remained normal when the lio+ transgene was expressed in these lio1 flies. Learning/memory remained normal when the lio+ transgene was expressed in wild-type (lio+) flies. The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Blotting, Southern
  • Chromosome Mapping
  • Cloning, Molecular*
  • DNA Probes
  • DNA Transposable Elements
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila / physiology
  • Drosophila Proteins*
  • Genes, Insect*
  • Hot Temperature
  • Larva / metabolism
  • Learning / physiology*
  • Memory / physiology
  • Molecular Sequence Data
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / physiology
  • Pupa / metabolism
  • Receptor Protein-Tyrosine Kinases*
  • Restriction Mapping
  • Smell


  • DNA Probes
  • DNA Transposable Elements
  • Drosophila Proteins
  • Proteins
  • pigeon protein, Drosophila
  • Receptor Protein-Tyrosine Kinases

Associated data

  • GENBANK/U32865