Peptide ligation and semisynthesis

Curr Opin Biotechnol. 1995 Aug;6(4):403-10. doi: 10.1016/0958-1669(95)80069-7.


Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destructive methods for peptide religation. Recently, a wide range of chemoselective ligation schemes have been elaborated that now permit the assembly of minimally protected peptides from either synthetic or natural sources.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Peptide Fragments / metabolism*
  • Protein Biosynthesis*
  • Protein Conformation
  • Protein Engineering / methods*
  • Proteins / chemical synthesis*
  • Proteins / chemistry
  • Thermodynamics


  • Peptide Fragments
  • Proteins