Use of vectorette and subvectorette PCR to isolate transgene flanking DNA

PCR Methods Appl. 1994 Oct;4(2):71-5. doi: 10.1101/gr.4.2.71.

Abstract

Vectorette PCR permits the specific amplification of DNA segments flanking a known DNA sequence. It enables the application of the PCR where sequence information is only available for one primer site. We now show that vectorette PCR can be used for the systematic mapping and retrieval of transgene flanking DNA. We also show that the sequence of large vectorette PCR fragments can be obtained without cloning, by the production of subvectorette fragments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA / isolation & purification*
  • DNA Primers
  • Genetic Vectors*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Restriction Mapping
  • Transgenes*

Substances

  • DNA Primers
  • DNA