Rat mitochondrial mtDNA-binding proteins to inter-specifically conserved sequences in the displacement loop region of vertebrate mtDNAs

Biochem Mol Biol Int. 1995 Aug;36(5):973-81.


We describe two new inter-specifically conserved sequences named Mt5 and Mt6 elements which are around the termination associated sequence (TAS) at the 3' end of the displacement loop (D-loop) region of vertebrate mtDNAs. We employed gel mobility shift assay to search for D-loop-binding proteins using partially purified rat mitochondrial mtDNA-binding proteins and 10 human DNA fragments covering the entire D-loop region as probe DNAs. Competition binding assays indicated the presence of four different rat mitochondrial D-loop-binding proteins. Each of the rat proteins bound, in a sequence-specific manner, to a probe DNA containing an inter-specifically conserved sequence; TAS, Mt5, B block, and a A+T-rich sequence overlapping the conserved sequence block I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding, Competitive
  • Conserved Sequence / genetics*
  • DNA Probes
  • DNA, Mitochondrial / genetics
  • DNA, Mitochondrial / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Mitochondria / metabolism
  • Molecular Sequence Data
  • Rats
  • Species Specificity


  • DNA Probes
  • DNA, Mitochondrial
  • DNA-Binding Proteins