Molecular comparison of the mitochondrial and cytoplasmic hsp70 of Trypanosoma cruzi, Trypanosoma brucei and Leishmania major

J Eukaryot Microbiol. Sep-Oct 1995;42(5):473-6. doi: 10.1111/j.1550-7408.1995.tb05893.x.

Abstract

We compared the expression and localization of the mitochondrial and cytoplasmic hsp70 of the protozoans Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. The mitochondrial protein is encoded by multiple mRNA in all species, while the cytoplasmic protein is encoded by a single mRNA. In all three species, the mitochondrial hsp70 is concentrated in the kinetoplast, a submitochondrial structure that houses the unusual DNA (kDNA) that characterizes this group of organisms, while the cytoplasmic protein is distributed throughout the cell. These results suggest that, in all kinetoplastid species, mt-hsp70 has a specific function in kDNA biology, possibly in the processes of kDNA replication, RNA editing or kinetoplast structure.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cytoplasm / chemistry*
  • HSP70 Heat-Shock Proteins / analysis*
  • HSP70 Heat-Shock Proteins / biosynthesis
  • Leishmania major / chemistry*
  • Leishmania major / metabolism
  • Mitochondria / chemistry*
  • Protozoan Proteins / analysis*
  • Protozoan Proteins / biosynthesis
  • RNA, Messenger / biosynthesis
  • RNA, Protozoan / biosynthesis
  • Trypanosoma / chemistry*
  • Trypanosoma brucei brucei / chemistry
  • Trypanosoma brucei brucei / metabolism
  • Trypanosoma cruzi / chemistry
  • Trypanosoma cruzi / metabolism

Substances

  • HSP70 Heat-Shock Proteins
  • Protozoan Proteins
  • RNA, Messenger
  • RNA, Protozoan