Simultaneous profile analysis of plasma amino and organic acids by capillary gas chromatography

J Chromatogr B Biomed Appl. 1995 Jul 21;669(2):352-7. doi: 10.1016/0378-4347(95)00115-y.


A simultaneous GC analysis of more than 20 amino and nearly 30 non-amino organic acids abundant in plasma is for the first time possible. Isolation of the analytes from the plasma matrix is not necessary, keto acids do not require a preliminary oximation. An instantaneous derivatization of the acids with ethyl chloroformate takes place directly in the medium after deproteinization. Less than 30 min are required to prepare a plasma sample for the GC analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / blood*
  • Chromatography, Gas / methods*
  • Keto Acids / blood*
  • Reference Standards
  • Reproducibility of Results


  • Amino Acids
  • Keto Acids