Induction and repression of alpha-amylase production in batch and continuous cultures of Aspergillus oryzae

Microbiology (Reading). 1995 Oct:141 ( Pt 10):2449-54. doi: 10.1099/13500872-141-10-2449.


The intra- and extracellular concentrations of alpha-amylase in Aspergillus oryzae have been measured during batch culture of a wild-type strain and two recombinant strains. The mean intracellular level for the two recombinant strains was about four to five times the level of the wild-type strain. The recombinant strains also had a higher alpha-amylase productivity, whereas the residence time of the intracellular alpha-amylase pool was approximately the same for the three strains. At high glucose concentrations there was a low constitutive synthesis of alpha-amylase, whereas at low glucose concentrations derepression resulted in an increased production rate. Shifts from a glucose- to a maltose-limited chemostat showed that maltose induces both the production and secretion of alpha-amylase. Finally, from immunoblots, both a glycosylated and an unglycosylated alpha-amylase have been detected.

MeSH terms

  • Amidohydrolases / metabolism
  • Aspergillus oryzae / enzymology*
  • Aspergillus oryzae / growth & development
  • Cell Division
  • Enzyme Induction
  • Enzyme Repression
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Fungal*
  • Glucose / metabolism
  • Glycosylation
  • Maltose / metabolism
  • Mycology / methods
  • Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
  • Starch / metabolism
  • alpha-Amylases / biosynthesis*


  • Maltose
  • Starch
  • alpha-Amylases
  • Amidohydrolases
  • Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
  • Glucose