Sixty new STSs (sequence-tagged sites) of human chromosome 21

H Tanahashi; T Ito; M Hattori; M Ohira; M Ohki; K Tashiro; Y Sakaki

doi:10.1093/dnares/1.2.85

Sixty new STSs (sequence-tagged sites) of human chromosome 21

DNA Res. 1994;1(2):85-9. doi: 10.1093/dnares/1.2.85.

Authors

H Tanahashi¹, T Ito, M Hattori, M Ohira, M Ohki, K Tashiro, Y Sakaki

Affiliation

¹ Human Genome Center, University of Tokyo, Japan.

PMID: 7584032
DOI: 10.1093/dnares/1.2.85

Abstract

From human chromosome 21-specific libraries, 22 SfiI linking clones and 38 P1 clones were isolated and regionally mapped on the chromosome. The terminal sequences of these clones were determined and pairs of PCR primers were generated which could specifically amplify the sequenced regions. These sequence-tagged sites (STSs) should be useful for constructing a high resolution map of human chromosome 21.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chromosomes, Human, Pair 21*
DNA Primers / chemistry
Deoxyribonucleases, Type II Site-Specific
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Restriction Mapping
Sequence Tagged Sites

Substances

DNA Primers
endodeoxyribonuclease SfiI
Deoxyribonucleases, Type II Site-Specific