Neutralization of HIV-1 by secretory IgA induced by oral immunization with a new macromolecular multicomponent peptide vaccine candidate

Nat Med. 1995 Jul;1(7):681-5. doi: 10.1038/nm0795-681.

Abstract

Control of pandemic infection of human immunodeficiency virus type 1 (HIV-1) requires some means of developing mucosal immunity against HIV-1 because sexual transmission of the virus occurs mainly through the mucosal tissues. However, there is no evidence as yet that the secretory immunoglobulin A (IgA) antibody induced by immunization with antigens in experimental animals can neutralize HIV-1. We demonstrate here that oral immunization with a new macromolecular peptide antigen and cholera toxin (CT) induces a high titre (1:2") of gut-associated and secretory IgA antibody to HIV-1. Using three different neutralizing assays, we clearly demonstrate that this secretory IgA antibody is able to neutralize HIV-1IIIB, HIV-1SF2 and HIV-1MN. Our new approach may prove to be important in the development of a mucosal vaccine that will provide protection of mucosal surfaces against HIV-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / administration & dosage
  • AIDS Vaccines / immunology*
  • Administration, Oral
  • Amino Acid Sequence
  • Animals
  • Antibody Specificity
  • Binding Sites
  • CD4 Antigens / metabolism
  • Cholera Toxin / immunology*
  • Consensus Sequence
  • Gastric Mucosa / immunology
  • HIV Antibodies / biosynthesis
  • HIV Antibodies / immunology*
  • HIV Envelope Protein gp120 / immunology*
  • HIV-1 / immunology*
  • Immunoglobulin A, Secretory / biosynthesis
  • Immunoglobulin A, Secretory / immunology*
  • Intestinal Mucosa / immunology
  • Japan
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neutralization Tests
  • Peptide Fragments / immunology*
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology*

Substances

  • AIDS Vaccines
  • CD4 Antigens
  • HIV Antibodies
  • HIV Envelope Protein gp120
  • HIV envelope protein gp120 (305-321)
  • Immunoglobulin A, Secretory
  • Peptide Fragments
  • Vaccines, Synthetic
  • Cholera Toxin