Macrophage inflammatory protein-1 beta: a C-C chemokine in osteoarthritis

Clin Immunol Immunopathol. 1995 Dec;77(3):307-14. doi: 10.1006/clin.1995.1157.


The aim of this study was to determine whether the cytokine macrophage inflammatory protein-1 beta (MIP-1 beta) is present and functionally active in the arthritic joint. We used immunoassays and bioassays to assess the presence and function of MIP-1 beta using samples obtained from 62 arthritic patients. MIP-1 beta levels were increased in synovial fluids (SFs) from patients with osteoarthritis (OA) (18.0 +/- 8.9 ng/ml) (SD) compared to patients with rheumatoid arthritis (RA) 6.1 +/- 2.9 ng/ml) or other forms of arthritis (10.4 +/- 7.0 ng/ml) (P < 0.05). Levels of OA SF MIP-1 beta were significantly greater than OA or normal serum levels of MIP-1 beta. Anti-MIP-1 beta neutralized 28% of the chemotactic activity for monocytes found in OA SFs. Isolated OA synovial tissue fibroblasts did not constitutively produce MIP-1 beta but could be induced to express this chemokine upon exposure to tumor necrosis factor-alpha, interleukin-1 beta, or lipopolysaccharide. Synovial tissue immunohistochemical staining revealed that the main immunopositive cells in OA were the lining cells as well as vascular smooth muscle and endothelial cells. A minority of macrophages were immunopositive as well. In this study, we identify MIP-1 beta as a unique cytokine increased in OA compared to RA SF. We conclude that MIP-1 beta may play a role in the ingress of monocytes into the OA joint.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arthritis / metabolism
  • Arthritis, Rheumatoid / metabolism*
  • Cells, Cultured
  • Chemokine CCL4
  • Chemokines / analysis
  • Chemotaxis, Leukocyte / physiology
  • Cytokines / analysis*
  • Cytokines / blood
  • Cytokines / immunology
  • Endothelium, Vascular / chemistry
  • Endothelium, Vascular / cytology
  • Fibroblasts / chemistry
  • Humans
  • Immune Sera / immunology
  • Immunoenzyme Techniques
  • Macrophage Inflammatory Proteins
  • Monocytes / physiology
  • Monokines / analysis*
  • Monokines / blood
  • Monokines / immunology
  • Muscle, Smooth, Vascular / chemistry
  • Muscle, Smooth, Vascular / cytology
  • Osteoarthritis / metabolism*
  • Synovial Fluid / chemistry*
  • Synovial Membrane / chemistry
  • Synovial Membrane / cytology


  • Chemokine CCL4
  • Chemokines
  • Cytokines
  • Immune Sera
  • Macrophage Inflammatory Proteins
  • Monokines